APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GTRAP41 , SCA5 , SCAR14 , SPTBN2 , spectrin beta, nonerythrocytic 2 , Spectrin Beta III , spectrin β III , spectrin beta, non-erythrocytic 2
Cellular Localization
Cytoplasm,Cytoskeleton,Cell cortex
Background
Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]
Database
Research Area
DATA IMAGES
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091](/templates/default/js/lightbox/images/loading.gif "Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091")
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GTX35091 FACS Image
FACS analysis of HeLa cells using GTX35091 SPTBN2 antibody [SPTBN2/1584].
Blue : Primary antibody
Red : Isotype control
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GTX35091 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX35091 ICC/IF Image
ICC/IF analysis of MeOH-fixed HeLa cells using GTX35091 SPTBN2 antibody [SPTBN2/1584].
Green : Primary antibody
Red : nucleus
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GTX35091 WB Image
WB analysis of HeLa cell lysate using GTX35091 SPTBN2 antibody [SPTBN2/1584].
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GTX35091 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX35091 SPTBN2 antibody [SPTBN2/1584].
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GTX35091 WB Image
WB analysis of PANC-1 cell lysate using GTX35091 SPTBN2 antibody [SPTBN2/1584].
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GTX35091 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX35091 SPTBN2 antibody [SPTBN2/1584].
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GTX35091 IHC-P Image
IHC-P analysis of rat pancreas tissue using GTX35091 SPTBN2 antibody [SPTBN2/1584].
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REFERENCE
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REVIEW
There are currently no reviews for SPTBN2 antibody [SPTBN2/1584] (GTX35091). Be the first to share your experience with this product.
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_FACS_187_w_23060801_214.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Protein Array (Protein Array). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_Protein-Array_2092_w_23060801_719.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Protein Array (Protein Array). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_ICC-IF_1588_w_23060801_768.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_WB_134_w_23060801_927.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_IHC-P_1624_w_23060801_487.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_WB_1651_w_23060801_946.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_IHC-P_895_w_23060801_915.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/small/GTX35091/GTX35091_20200115_IHC-P_1825_w_23060801_134.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_FACS_187_w_23060801_214.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Flow cytometry (FACS). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Protein Array (Protein Array). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_Protein-Array_2092_w_23060801_719.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Protein Array (Protein Array). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_ICC-IF_1588_w_23060801_768.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_WB_134_w_23060801_927.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_IHC-P_1624_w_23060801_487.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_WB_1651_w_23060801_946.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in Western Blot (WB). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_IHC-P_895_w_23060801_915.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")
![Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091](/upload/website/prouct_img/normal/GTX35091/GTX35091_20200115_IHC-P_1825_w_23060801_134.webp "Anti-SPTBN2 antibody [SPTBN2/1584] used in IHC (Paraffin sections) (IHC-P). GTX35091")