APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2 μg/ml |
| 1-2 μg/ml |
| 1-2 μg/million cells |
| 2-4 μg/ml (for coating) |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human SPTBN2 fragment (aa356-475)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
spectrin beta, non-erythrocytic 2 , GTRAP41 , SCA5 , SCAR14
Cellular Localization
Cytoplasm, cytoskeleton
Background
Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]
Database
Research Area
DATA IMAGES
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716](/templates/default/js/lightbox/images/loading.gif "Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716")
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GTX02716 IHC-P Image
IHC-P analysis of human pancreatic cancer section using GTX02716 SPTBN2 antibody [SPTBN2/2894R].
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GTX02716 FACS Image
FACS analysis of HeLa cells using GTX02716 SPTBN2 antibody [SPTBN2/2894R].
Blue : Primary antibody
Red : Isotype control
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GTX02716 WB Image
WB analysis of PANC-1 whole cell lysate using GTX02716 SPTBN2 antibody [SPTBN2/2894R].
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GTX02716 WB Image
WB analysis of HeLa whole cell lysate using GTX02716 SPTBN2 antibody [SPTBN2/2894R].
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GTX02716 IHC-P Image
IHC-P analysis of human pancreatic cancer section using GTX02716 SPTBN2 antibody [SPTBN2/2894R].
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REFERENCE
There are currently no references for SPTBN2 antibody [SPTBN2/2894R] (GTX02716). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SPTBN2 antibody [SPTBN2/2894R] (GTX02716). Be the first to share your experience with this product.
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716](/upload/website/prouct_img/small/GTX02716/GTX02716_20210319_IHC-P_1_w_23053122_785.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Flow cytometry (FACS). GTX02716](/upload/website/prouct_img/small/GTX02716/GTX02716_20210319_FACS_w_23053122_891.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Flow cytometry (FACS). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716](/upload/website/prouct_img/small/GTX02716/GTX02716_20210319_WB_1_w_23053122_554.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716](/upload/website/prouct_img/small/GTX02716/GTX02716_20210319_WB_w_23053122_924.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716](/upload/website/prouct_img/small/GTX02716/GTX02716_20210319_IHC-P_w_23053122_697.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716](/upload/website/prouct_img/normal/GTX02716/GTX02716_20210319_IHC-P_1_w_23053122_785.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Flow cytometry (FACS). GTX02716](/upload/website/prouct_img/normal/GTX02716/GTX02716_20210319_FACS_w_23053122_891.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Flow cytometry (FACS). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716](/upload/website/prouct_img/normal/GTX02716/GTX02716_20210319_WB_1_w_23053122_554.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716](/upload/website/prouct_img/normal/GTX02716/GTX02716_20210319_WB_w_23053122_924.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in Western Blot (WB). GTX02716")
![Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716](/upload/website/prouct_img/normal/GTX02716/GTX02716_20210319_IHC-P_w_23053122_697.webp "Anti-SPTBN2 antibody [SPTBN2/2894R] used in IHC (Paraffin sections) (IHC-P). GTX02716")