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SPTLC1 antibody

Anti-SPTLC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32011
Anti-SPTLC1 antibody used in Western Blot (WB). GTX32011

Cat No. GTX32011

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 Cell Lysate(GTX27910) , Human Lung Tissue Slide , HeLa Cell Lysate

Specificity/Sensitivity

At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SPTLC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPTLC1.The immunogen is located within amino acids 380 - 430 of SPTLC1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serine palmitoyltransferase long chain base subunit 1 , HSAN1 , HSN1 , LBC1 , LCB1 , SPT1 , SPTI

Cellular Localization

Endoplasmic reticulum membrane

Background

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-SPTLC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32011

GTX32011 IHC-P Image

IHC-P analysis of human lung tissue using GTX32011 SPTLC1 antibody.
Working concentration : 5 μg/ml

Anti-SPTLC1 antibody used in Western Blot (WB). GTX32011

GTX32011 WB Image

WB analysis of (A) A549 and (B) HeLa cell lysate using GTX32011 SPTLC1 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 339