APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A549 Cell Lysate(GTX27910) , Human Lung Tissue Slide , HeLa Cell Lysate
Product Note
At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
SPTLC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPTLC1.The immunogen is located within amino acids 380 - 430 of SPTLC1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine palmitoyltransferase long chain base subunit 1 , HSAN1 , HSN1 , LBC1 , LCB1 , SPT1 , SPTI
Cellular Localization
Endoplasmic reticulum membrane
Background
Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX32011 WB Image
WB analysis of (A) A549 and (B) HeLa cell lysate using GTX32011 SPTLC1 antibody.
Working concentration : 1 μg/ml
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GTX32011 IHC-P Image
IHC-P analysis of human lung tissue using GTX32011 SPTLC1 antibody.
Working concentration : 5 μg/ml
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REFERENCE
There are currently no references for SPTLC1 antibody (GTX32011). Be the first to share your publications with this product.
REVIEW
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