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SQSTM1 / P62 antibody [GT1193]

Recombinant antibody KO/KD Orthogonal
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunoprecipitation (IP). GTX00955

Cat. No. GTX00955

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1193

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SQSTM1 / P62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SQSTM1 / p62.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sequestosome 1 , A170 , FTDALS3 , OSIL , PDB3 , SQSTM1 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm,Cytosol,Late endosome,Lysosome,Cytoplasmic vesicle,Autophagosome,Nucleus,Endoplasmic reticulum,PML body,Myofibril,Sarcomere

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [GT1193] (GTX00955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

KO/KD

WB analysis of wild-type (WT) and SQSTM1 / p62 knockout (KO) 293T cell extracts using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:1000
Laoding : 25μg

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

WB analysis of various samples using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:1000
Loading : 25μg

Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955

GTX00955 IHC-P Image

IHC-P analysis of Rat spleen tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955

GTX00955 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00955 SQSTM1 / P62 antibody [GT1193].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955

GTX00955 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955

GTX00955 ICC/IF Image

ICC/IF analysis of PFA-fixed L929 cells using GTX00955 SQSTM1 / P62 antibody [GT1193].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunoprecipitation (IP). GTX00955

GTX00955 IP Image

IP analysis of HeLa cell lysate using GTX00955 SQSTM1 / P62 antibody [GT1193].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6) : 2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399