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SQSTM1 / P62 antibody [GT381]

KO/KD Orthogonal
Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889
Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889
Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889
Anti-SQSTM1 / P62 antibody [GT381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629889
Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889
Anti-SQSTM1 / P62 antibody [GT381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629889

Cat. No. GTX629889

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT381

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SQSTM1 / P62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A549 , H1299 , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm , Late endosome , Nucleus

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889

GTX629889 WB Image

Orthogonal

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hr and 24hr) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889

GTX629889 WB Image

Orthogonal

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889

GTX629889 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500.

Anti-SQSTM1 / P62 antibody [GT381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629889

GTX629889 ICC/IF Image

SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
Anti-SQSTM1 / P62 antibody [GT381] used in Western Blot (WB). GTX629889

GTX629889 WB Image

SQSTM1 antibody [GT381] detects SQSTM1 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
10 % SDS-PAGE
SQSTM1 antibody [GT381] (GTX629889) dilution: 1:1000

Anti-SQSTM1 / P62 antibody [GT381] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629889

GTX629889 ICC/IF Image

SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations