APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888177
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B
Cellular Localization
Cytoplasm , Late endosome , Nucleus
Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX629889 WB Image
SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hr and 24hr) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000. The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
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GTX629889 WB Image
SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000. The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
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GTX629889 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500.
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GTX629889 ICC/IF Image
SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
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GTX629889 WB Image
SQSTM1 antibody [GT381] detects SQSTM1 protein by Western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract 10 % SDS-PAGE SQSTM1 antibody [GT381] (GTX629889) dilution: 1:1000
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GTX629889 ICC/IF Image
SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min . Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500. Blue: Hoechst 33342 staining.
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REFERENCE
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REVIEW
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