SQSTM1 / P62 antibody [HL1113]

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.