APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909970
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B
Cellular Localization
Cytoplasm, cytosol,Lysosome,Nucleus,Endoplasmic reticulum
Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/templates/default/js/lightbox/images/loading.gif "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
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GTX636328 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636328 ICC/IF Image
SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636328 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636328 IHC-P Image
SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636328 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636328 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636328 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for SQSTM1 / P62 antibody [HL1113] (GTX636328). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SQSTM1 / P62 antibody [HL1113] (GTX636328). Be the first to share your experience with this product.
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20220812_WB_competitor_watermark_22081423_370.webp "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20220121_ICC_IF_22020818_125.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20211029_WB_treatment_Thapsigargin_21110220_376.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20211029_IHC-P_21110220_844.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20220318_WB_M_22032223_672.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20220506_WB_M_treatment_Thapsigargin_22051120_213.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/small/GTX636328/GTX636328_44452_20220617_WB_R_22062121_612.webp "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220812_WB_competitor_watermark_22081423_370.webp "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220121_ICC_IF_22020818_125.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20211029_WB_treatment_Thapsigargin_21110220_376.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20211029_IHC-P_21110220_844.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220318_WB_M_22032223_672.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220506_WB_M_treatment_Thapsigargin_22051120_213.jpg "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")
![Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328](/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220617_WB_R_22062121_612.webp "Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328")