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SQSTM1 / P62 antibody

Anti-SQSTM1 / P62 antibody used in Western Blot (WB). GTX31795
Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795
Anti-SQSTM1 / P62 antibody used in Western Blot (WB). GTX31795
Anti-SQSTM1 / P62 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31795
Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795
Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795
Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795
Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

Cat No. GTX31795

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

K562 Cell Lysate(GTX27911) , Human Spleen Tissue Lysate(GTX27921)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.The immunogen corresponding to a region within amino acids 391 and 440 of human SQSTM1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm, cytosol,Lysosome,Nucleus,Endoplasmic reticulum

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody used in Western Blot (WB). GTX31795

GTX31795 WB Image

WB analysis of human spleen tissue lysate using GTX31795 SQSTM1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

GTX31795 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml

Anti-SQSTM1 / P62 antibody used in Western Blot (WB). GTX31795

GTX31795 WB Image

WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31795 SQSTM1 antibody.
Working concentration : 1 μg/ml

Anti-SQSTM1 / P62 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31795

GTX31795 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml

Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

GTX31795 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 2 μg/ml

Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

GTX31795 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 5 μg/ml

Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

GTX31795 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 5 μg/ml

Anti-SQSTM1 / P62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31795

GTX31795 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399