APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| Assay dependent |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
K562 Cell Lysate(GTX27911) , Human Spleen Tissue Lysate(GTX27921)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.The immunogen corresponding to a region within amino acids 391 and 440 of human SQSTM1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B
Cellular Localization
Cytoplasm, cytosol,Lysosome,Nucleus,Endoplasmic reticulum
Background
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
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GTX31795 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 2 μg/ml
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GTX31795 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml
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GTX31795 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 5 μg/ml
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GTX31795 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 5 μg/ml
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GTX31795 ICC/IF Image
ICC/IF analysis of A431 cells using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml
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GTX31795 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX31795 SQSTM1 antibody.
Working concentration : 20 μg/ml
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GTX31795 WB Image
WB analysis of human spleen tissue lysate using GTX31795 SQSTM1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31795 WB Image
WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31795 SQSTM1 antibody.
Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for SQSTM1 / P62 antibody (GTX31795). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SQSTM1 / P62 antibody (GTX31795). Be the first to share your experience with this product.
