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SQSTM1 / P62 (phospho Ser403) antibody

GTX128171_41346_CT_WB_MG132_18121410_897.jpg
GTX128171_41570_IHC_R_18121410_930.jpg
GTX128171_41570_20160630_WB_Thapsigargin_18121410_644.jpg
GTX128171_41346_20150212_FACS_18121410_358.jpg
GTX128171_41346_IHC_18121410_627.jpg
GTX128171_43110_20180316_WB_treatment_Thapsigargin_18121410_111.jpg
GTX128171_43194_20181205_ICC_IF_Thapsigargin_19021719_599.jpg

Cat No. GTX128171

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HepG2 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3μM Thapsigargin treatment for 12hr and 24hr) , Huh7 whole cell lysate/extract (5 μM MG132) , Huh7 whole cell lysate/extract (10 μM MG132)

Predict Reactivity

Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human SQSTM1 (phospho Ser403). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sequestosome 1,A170,Dmrv,Ftdals3,Nadgp,Osil,Pdb3,Zip3,P60,P62,P62B,Sqstm1

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128171_41346_CT_WB_MG132_18121410_897.jpg

GTX128171 WB Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein by western blot analysis.
A. 30 µg Huh7 whole cell lysate/extract (untreated)
B. 30 µg Huh7 whole cell lysate/extract (5 µM MG132)
C. 30 µg Huh7 whole cell lysate/extract (untreated)
D. 30 µg Huh7 whole cell lysate/extract (10 µM MG132)
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128171_41570_IHC_R_18121410_930.jpg

GTX128171 IHC-P Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128171_41570_20160630_WB_Thapsigargin_18121410_644.jpg

GTX128171 WB Image

HepG2 cells were untreated or treated with 3μM thapsigargin for 12 and 24 hrs. Whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 (phospho Ser403) antibody (GTX128171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128171_41346_20150212_FACS_18121410_358.jpg

GTX128171 FACS Image

SQSTM1 (phospho Ser403) antibody (GTX128171) detects SQSTM1 (phospho Ser403) protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

GTX128171_41346_IHC_18121410_627.jpg

GTX128171 IHC-P Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128171_43110_20180316_WB_treatment_Thapsigargin_18121410_111.jpg

GTX128171 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128171_43194_20181205_ICC_IF_Thapsigargin_19021719_599.jpg

GTX128171 ICC/IF Image

SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

SQSTM1 / P62 (phospho Ser403) antibody Cat No. GTX128171

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : NG108-15 cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1.5Hr
Date : Anonymous submitted on 08-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 25μg
Blocking : 5% BSA, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169