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SQSTM1 / P62 (phospho Ser403) antibody

Orthogonal
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in IHC (Paraffin sections) (IHC-P). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Flow cytometry (FACS). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in IHC (Paraffin sections) (IHC-P). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

Cat. No. GTX128171

Reference 19
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SQSTM1 / P62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Huh7 (5 μM MG132) , Huh7 (10 μM MG132) , HepG2 (3μM Thapsigargin treatment for 12hr and 24hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Zebrafish, Sheep, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser403 of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885723

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in IHC (Paraffin sections) (IHC-P). GTX128171

GTX128171 IHC-P Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Flow cytometry (FACS). GTX128171

GTX128171 FACS Image

SQSTM1 (phospho Ser403) antibody (GTX128171) detects SQSTM1 (phospho Ser403) protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in IHC (Paraffin sections) (IHC-P). GTX128171

GTX128171 IHC-P Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract (untreated)
B. 30 μg Huh7 whole cell lysate/extract (5 μM MG132)
C. 30 μg Huh7 whole cell lysate/extract (untreated)
D. 30 μg Huh7 whole cell lysate/extract (10 μM MG132)
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

Orthogonal

HepG2 cells were untreated or treated with 3μM thapsigargin for 12 and 24 hrs. Whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 (phospho Ser403) antibody (GTX128171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128171

GTX128171 ICC/IF Image

Orthogonal

SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

Orthogonal

The data was published in the journal Physiol Rep in 2017. PMID: 29263116

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

The data was published in the 2019 in Cell Death Dis. PMID: 31320609

Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171

GTX128171 WB Image

The data was published in the journal Physiol Rep in 2017. PMID: 29263116

REFERENCE

Application Reference

Ikeda R et al. EMBO J 2023; 42 (14) : e113349 Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.
Porras G et al. Int J Mol Sci 2023; 24 (3) Functional Characterization of a Familial ALS-Associated Missense TBK1 (p-Arg573Gly) Mutation in Patient-Derived Lymphoblasts.
Application : WB
Reactivity : Human
Yi Liu et al. Genes Dis 2021; 8 (2) : 232-240 Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.
Application : WB
Reactivity : Human
Kageyama S et al. Nat Commun 2021; 12 (1) : 16 p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response.
Application : WB
Reactivity : Mouse
El-Derany MO et al. Biochem Pharmacol 2020; 177 : 113942 Pyrvinium pamoate attenuates non-alcoholic steatohepatitis: Insight on hedgehog/Gli and Wnt/β-catenin signaling crosstalk.
Application : WB
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

SQSTM1 / P62 (phospho Ser403) antibody Cat. No. GTX128171

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : NG108-15 cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1.5Hr
Anti-SQSTM1 / P62 (phospho Ser403) antibody used in Western Blot (WB). GTX128171
Date : Anonymous submitted on 08-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 25μg
Blocking : 5% BSA, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 18Hr
Submit a Review
Application Reference
Ikeda R et al. EMBO J 2023; 42 (14):e113349 Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.
Porras G et al. Int J Mol Sci 2023; 24 (3) Functional Characterization of a Familial ALS-Associated Missense TBK1 (p-Arg573Gly) Mutation in Patient-Derived Lymphoblasts.
Application : WB
Reactivity : Human
Yi Liu et al. Genes Dis 2021; 8 (2):232-240 Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway.
Application : WB
Reactivity : Human
Kageyama S et al. Nat Commun 2021; 12 (1):16 p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response.
Application : WB
Reactivity : Mouse
El-Derany MO et al. Biochem Pharmacol 2020; 177:113942 Pyrvinium pamoate attenuates non-alcoholic steatohepatitis: Insight on hedgehog/Gli and Wnt/β-catenin signaling crosstalk.
Application : WB
Reactivity : Rat
Bondad SEC et al. Biol Trace Elem Res 2020; (Epub) Analysis of Cadmium, Epigallocatechin Gallate, and Vitamin C Co-exposure on PC12 Cellular Mechanisms.
Application : WB
Reactivity : Rat
Lee S et al. Autophagy 2019;:1-17 PTK2/FAK regulates UPS impairment via SQSTM1/p62 phosphorylation in TARDBP/TDP-43 proteinopathies.
Application : WB
Reactivity : Mouse
Qiu Y et al. Cell Death Dis 2019; 10 (8):547 Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis.
Application : WB
Reactivity : Mouse
Ding X et al. Autophagy 2019;:1月13日 RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.
Application : WB
Reactivity : Human
Kita-Tomihara T et al. Int J Food Sci Nutr 2019;:1-11 Polyphenol-enriched azuki bean (Vina angularis) extract reduces the oxidative stress and prevents DNA oxidation in the hearts of streptozotocin-induced early diabetic rats.
Application : WB
Reactivity : Rat
Catanese A et al. Autophagy 2019;:1-19 Retinoic acid worsens ATG10-dependent autophagy impairment in TBK1-mutant hiPSC-derived motoneurons through SQSTM1/p62 accumulation.
Application : WB
Reactivity : Human
Yao J et al. Autophagy 2018; 14 (7):1226-1238 Inhibiting autophagy reduces retinal degeneration caused by protein misfolding.
Yao J et al. Autophagy 2018;:1月6日 Autophagosome immunoisolation from GFP-LC3B mouse tissue.
Application : WB
Reactivity : Mouse
Shinrye Lee et al. bioRxiv 2018; (Epub) PTK2 regulates the UPS impairment via p62 phosphorylation in TDP-43 proteinopathy.
Application : WB, ICC/IF
Reactivity : Human
Endo H et al. Sci Rep 2018; 8 (1):10122 Glucose starvation induces LKB1-AMPK-mediated MMP-9 expression in cancer cells.
Application : WB
Reactivity : Human
Zeng N et al. Eur J Appl Physiol 2018; (Epub) The putative leucine sensor Sestrin2 is hyperphosphorylated by acute resistance exercise but not protein ingestion in human skeletal muscle.
Application : WB
Reactivity : Human
Jiang X et al. Oncogene 2017; 36 (50):6850-6862 VPS34 stimulation of p62 phosphorylation for cancer progression.
Zeng N et al. Physiol Rep 2017; 5 (24) Acute resistance exercise induces Sestrin2 phosphorylation and p62 dephosphorylation in human skeletal muscle.
Application : WB
Reactivity : Human
Watanabe Y et al. Autophagy 2016;:0 HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis.
Application : WB, ICC/IF, IHC
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169