GeneTex
United States (US)

SR-BI + SR-BII antibody

GTX30630_33_FACS_18121410_362.jpg
GTX30630_1438_WB_18121410_179.jpg
GTX30630_563_IHC-P_18121410_442.jpg
GTX30630_218_ICC-IF_18121410_571.jpg

Cat No. GTX30630

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50 - 1:1000
IHC-P Assay dependent
FACS 1:400
IP Assay dependent
IHC 1:10 - 1:500
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Immunogen

A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII and SR-BI are identical except for the encoded c-terminal cytoplasmic domain. Both SR-BI and SR-BII bind HDL and mediates selective uptake of HDL cholesteryl ester, but with SR-BII having an approximately 4-fold lower efficiency than SR-BI. SR-BI and SR-BII are expressed primarily in liver and non-placental steroidgenic tissues.Although the role of these scavenger receptors is not completely clear, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with both isoforms mediating selective transfer of lipid between HDL and cells. Therefore, the relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
DATA IMAGES
GTX30630_33_FACS_18121410_362.jpg

GTX30630 FACS Image

FACS analysis of NIH-3T3 cells using GTX30630 SR-BI + SR-BII antibody. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

GTX30630_1438_WB_18121410_179.jpg

GTX30630 WB Image

WB analysis of mouse liver tissue lysate using GTX30630 SR-BI + SR-BII antibody.
Lane 1: wild-type mice
Lane 2: SR-BI deficient mice
Loading : 80μg

GTX30630_563_IHC-P_18121410_442.jpg

GTX30630 IHC-P Image

IHC-P analysis of human liver tissue using GTX30630 SR-BI + SR-BII antibody.
Dilution : 5 μg/ml

GTX30630_218_ICC-IF_18121410_571.jpg

GTX30630 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30630 SR-BI + SR-BII antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Package List Price ($)
$ 339