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SR-BI antibody

Orthogonal
Anti-SR-BI antibody used in IHC (Paraffin sections) (IHC-P). GTX113645
Anti-SR-BI antibody used in Western Blot (WB). GTX113645
Anti-SR-BI antibody used in IHC (Paraffin sections) (IHC-P). GTX113645
Anti-SR-BI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113645
Anti-SR-BI antibody used in Western Blot (WB). GTX113645
Anti-SR-BI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113645
Anti-SR-BI antibody used in Western Blot (WB). GTX113645

Cat. No. GTX113645

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SR-BI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

rat liver

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SR-BI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037943

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scavenger receptor class B member 1 , CD36L1 , CLA-1 , CLA1 , HDLQTL6 , SR-BI , SRB1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Membrane , caveola; Multi-pass membrane protein

Background

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.

Database

Research Area

DATA IMAGES

Anti-SR-BI antibody used in IHC (Paraffin sections) (IHC-P). GTX113645

GTX113645 IHC-P Image

SR-BI antibody detects SR-BI protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SR-BI antibody (GTX113645) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SR-BI antibody used in Western Blot (WB). GTX113645

GTX113645 WB Image

SR-BI antibody detects SR-BI protein by western blot analysis.
A. 50 μg rat liver extract
7.5% SDS-PAGE
SR-BI antibody (GTX113645) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SR-BI antibody used in IHC (Paraffin sections) (IHC-P). GTX113645

GTX113645 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using SR-BI(GTX113645) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SR-BI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113645

GTX113645 ICC/IF Image

SR-BI antibody detects SR-BI protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SR-BI protein stained by SR-BI antibody (GTX113645) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-SR-BI antibody used in Western Blot (WB). GTX113645

GTX113645 WB Image

Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SR-BI antibody (GTX113645) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SR-BI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113645

GTX113645 ICC/IF Image

SR-BI antibody detects SR-BI protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SR-BI protein stained by SR-BI antibody (GTX113645) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-SR-BI antibody used in Western Blot (WB). GTX113645

GTX113645 WB Image

The data was published in the 2022 in Mol Med. PMID: 35236285

REFERENCE

Application Reference

Zhiwen Wang et al. Mol Med 2022; 28 (1) : 26 The LOX-1 receptor ectopically expressed in the liver alleviates atherosclerosis by clearing Ox-LDL from the circulation.
Application : WB
Reactivity : Mouse
Wang B et al. J Pharm Sci 2014; 103 (12) : 3994-4004 combination of apolipoprotein a1-modi liposome-* with autophagy inhibitors overcame drug resistance in vitro.
Application : WB
Reactivity : Human
Dorner M et al. Nature 2013; 501 (7466) : 237-41 Completion of the entire hepatitis C virus life cycle in genetically humanized mice.
Application : IHC-P
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169