GeneTex
United States (US)

SRC1 antibody [1135/H4]

GTX15795_1543_WB_19011519_238.jpg
GTX15795_1542_WB_19011519_503.jpg

Cat No. GTX15795

Host Mouse
Clonality Monoclonal
Clone Name 1135/H4
Isotype IgG1
Application WB, IHC-P, FACS, IP, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
IHC-P 1:200
FACS 1μg per 10⁶ cells
IP Assay dependent
ChIP assay 5μl/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

Specificity/Sensitivity

The epitope for GTX15795 has been mapped to the extreme C-terminal end of this fragment.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues 477-947 of human SRC-1.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Coactivator 1,F-Src-1,Kat13A,Rip160,Src1,Bhlhe42,Bhlhe74,Ncoa1

Cellular Localization

Nucleus

Background

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15795_1543_WB_19011519_238.jpg

GTX15795 WB Image

WB analysis of COS cells using GTX15795 SRC1 antibody [1135/H4].

GTX15795_1542_WB_19011519_503.jpg

GTX15795 WB Image

WB analysis of 25 ug of LNCaP (lane 1) and PC-3 (lane 2) using GTX15795 SRC1 antibody [1135/H4].
Dilution : 1:2000

Package List Price ($)
$ 319