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SRC3 antibody [N1N2], N-term

Anti-SRC3 antibody [N1N2], N-term used in Western Blot (WB). GTX113869
Anti-SRC3 antibody [N1N2], N-term used in Western Blot (WB). GTX113869
Anti-SRC3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113869
Anti-SRC3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113869

Cat. No. GTX113869

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SRC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SRC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036189

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor coactivator 3 , ACTR , AIB-1 , AIB1 , CAGH16 , CTG26 , KAT13B , RAC3 , SRC-3 , SRC3 , TNRC14 , TNRC16 , TRAM-1 , bHLHe42 , pCIP

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SRC3 antibody [N1N2], N-term used in Western Blot (WB). GTX113869

GTX113869 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with SRC3 antibody [N1N2], N-term (GTX113869) diluted at 1:2000 and competitor's antibody (SC-7216) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SRC3 antibody [N1N2], N-term used in Western Blot (WB). GTX113869

GTX113869 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX113869 diluted at 1:2000

Anti-SRC3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113869

GTX113869 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using NCOA3(GTX113869) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SRC3 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113869

GTX113869 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SRC3 antibody [N1N2], N-term (GTX113869) at 1:200 dilution.

REFERENCE

Application Reference

Liu G et al. Oncogene 2018; (Epub) Lung fibroblasts promote metastatic colonization through upregulation of stearoyl-CoA desaturase 1 in tumor cells.
Application : WB
Reactivity : Human
Dagdemir A et al. Cancer Genomics Proteomics 2016; 13 (4) : 291-303 Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169