APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Hamster(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 349-378 amino acids from the Central region of human SREBF1.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sterol regulatory element binding transcription factor 1 , SREBP1 , bHLHd1
Cellular Localization
Endoplasmic reticulum membrane,Golgi apparatus membrane
Background
This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]
Database
Research Area
DATA IMAGES
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GTX81061 WB Image
WB analysis of MDA-MB435 cell lysate (35ug/lane) using GTX81061 SREBP1 antibody, Internal.
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GTX81061 WB Image
WB analysis of Jurkat whole cell lysate using GTX81061 SREBP1 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:1000
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GTX81061 FACS Image
FACS analysis of Ramos cells using GTX81061 SREBP1 antibody, Internal.
Top histogram : negative control
Bottom histogram : Ramos cells
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GTX81061 IHC-P Image
IHC-P analysis of human breast carcinoma using GTX81061 SREBP1 antibody, Internal.
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REFERENCE
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REVIEW
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