APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Daudi Cell Lysate(GTX23951) , Human Spleen Tissue Slide
Product Note
At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
SREBP1 antibody was raised against a 17 amino acid peptide near the center of human SREBP1.The immunogen is located within amino acids 450 - 500 of SREBP1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sterol regulatory element binding transcription factor 1 , SREBP1 , bHLHd1
Cellular Localization
Endoplasmic reticulum membrane,Golgi apparatus membrane
Background
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31579 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31579 SREBP1 antibody.
Working concentration : 5 μg/ml
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GTX31579 WB Image
WB analysis of Daudi cell lysate using GTX31579 SREBP1 antibody.
Working concentration : 1 μg/ml
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GTX31579 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31579 SREBP1 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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