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SREBP1 antibody

Orthogonal
Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299
Anti-SREBP1 antibody used in Western Blot (WB). GTX79299
Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299
Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299
Anti-SREBP1 antibody used in Western Blot (WB). GTX79299

Cat. No. GTX79299

Reference 12

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SREBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:50-1:500
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11168147

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sterol regulatory element binding transcription factor 1 , ADD1 , SREBP1 , bHLHd1

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Alternatively spliced transcript variants have been characterized for this gene. [provided by RefSeq, Nov 2017]

Database

Research Area

DATA IMAGES

Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299

GTX79299 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX79299 SREBP1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-SREBP1 antibody used in Western Blot (WB). GTX79299

GTX79299 WB Image

WB analysis of various samples using GTX79299 SREBP1 antibody.
Lane 1: mouse liver lysate
Lane 2: rat liver lysate
Loading: 25 μg per lane
Dilution: 1:1000

Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299

GTX79299 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX79299 SREBP1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-SREBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79299

GTX79299 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX79299 SREBP1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-SREBP1 antibody used in Western Blot (WB). GTX79299

GTX79299 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019.PMID: 31614639

REFERENCE

Application Reference

Huang HC et al. Nutrients 2023; 15 (20) Partial Replacement of Diet with Dehulled Adlay Ameliorates Hepatic Steatosis, Inflammation, Oxidative Stress, and Gut Dysbiosis in Rats with Nonalcoholic Fatty Liver Disease.
Hee SW et al. iScience 2023; 26 (10) : 107997 15-keto-PGE(2) alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factors.
Lin KY et al. Curr Res Food Sci 2023; 7 : 100546 Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats.
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Garc?a-Arroyo FE et al. Int J Mol Sci 2021; 22 (5) Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Huang HC et al. Nutrients 2023; 15 (20) Partial Replacement of Diet with Dehulled Adlay Ameliorates Hepatic Steatosis, Inflammation, Oxidative Stress, and Gut Dysbiosis in Rats with Nonalcoholic Fatty Liver Disease.
Hee SW et al. iScience 2023; 26 (10):107997 15-keto-PGE(2) alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factors.
Lin KY et al. Curr Res Food Sci 2023; 7:100546 Caulerpa lentillifera improves ethanol-induced liver injury and modulates the gut microbiota in rats.
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Garc?a-Arroyo FE et al. Int J Mol Sci 2021; 22 (5) Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet.
Application : WB
Reactivity : Mouse
Yeh WJ et al. Nutrients 2020; 12 (12) Hylocereus polyrhizus Peel Extract Retards Alcoholic Liver Disease Progression by Modulating Oxidative Stress and Inflammatory Responses in C57BL/6 Mice.
Application : WB
Reactivity : Human
Chang TC et al. BMC Complement Altern Med 2019; 19 (1):368 Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice.
Application : WB
Reactivity : Human
Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
Application : WB
Chen JR et al. J Food Sci 2019; (Epub) Antroquinonol Attenuated Abdominal and Hepatic Fat Accumulation in Rats Fed an Obesogenic Diet.
Application : WB
Reactivity : Rat
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
Application : WB
Reactivity : Human
Grossini E et al. J Endocrinol 2014; 222 (1):137-49 Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig.
Application : WB
Reactivity : Mouse
Lai YS et al. J Agric Food Chem 2014; 62 (25):5897-906 Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399