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SREBP2 antibody [N1N3]

Anti-SREBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105537
Anti-SREBP2 antibody [N1N3] used in Western Blot (WB). GTX105537

Cat No. GTX105537

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SREBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sterol regulatory element binding transcription factor 2 , SREBP-2 , SREBP2 , bHLHd2

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cytoplasmic vesicle , COPII-coated vesicle membrane; Multi-pass membrane protein , Processed sterol regulatory element-binding protein 2:

Background

This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SREBP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105537

GTX105537 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SREBF2(GTX105537) antibody at 1:500 dilution.

Anti-SREBP2 antibody [N1N3] used in Western Blot (WB). GTX105537

GTX105537 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX105537 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169