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SREBP2 antibody

Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865
Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865
Anti-SREBP2 antibody used in Western Blot (WB). GTX82865
Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865

Cat. No. GTX82865

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SREBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 1:20-1:200
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_626055

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sterol regulatory element binding transcription factor 2 , SREBP-2 , SREBP2 , Srebf2_retired

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

transcription factor; may be involved in spermatogenesis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865

GTX82865 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX82865 SREBP2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865

GTX82865 ICC/IF Image

ICC/IF analysis of L6 cells using GTX82865 SREBP2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-SREBP2 antibody used in Western Blot (WB). GTX82865

GTX82865 WB Image

WB analysis of 25 ug of mouse liver (Lane 1), rat liver (Lane 2) and HepG2 (Lane 3) cell lysates using GTX82865 SREBP2 antibody.
Dilution : 1:500

Anti-SREBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82865

GTX82865 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82865 SREBP2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

Application Reference

Irene Ferrarese et al. J. Funct. Foods 2023; 108 : 105724 Bergamot (Citrus bergamia) peel extract as new hypocholesterolemic agent modulating PCSK9 expression
Application : WB
Reactivity : Human
Kang J et al. Proc Natl Acad Sci U S A 2015; 112 (31) : E4236-45 Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399