GeneTex
United States (US)

SRF antibody [N1C1]

GTX101043_40073_IFA_19011506_248.jpg
GTX101043_40073_IHC_M_2_19011506_966.jpg
GTX101043_40073_IHC_M_19011506_740.jpg
GTX101043_WB_19011506_883.jpg

Cat No. GTX101043

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SRF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MCM1 antibody, SRF antibody, serum response factor antibody, serum response factor (c-fos serum response element-binding transcription factor) antibody

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101043_40073_IFA_19011506_248.jpg

GTX101043 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SRF(GTX101043) antibody at 1:500 dilution.

GTX101043_40073_IHC_M_2_19011506_966.jpg

GTX101043 IHC-P Image

SRF antibody [N1C1] detects SRF protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
SRF antibody [N1C1] (GTX101043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101043_40073_IHC_M_19011506_740.jpg

GTX101043 IHC-P Image

SRF antibody [N1C1] detects SRF protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
SRF antibody [N1C1] (GTX101043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101043_WB_19011506_883.jpg

GTX101043 WB Image

Sample (30 µg of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX101043 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf