GeneTex
  • Country / Location Selection

United States (US)

SRP1 antibody

Anti-SRP1 antibody used in Western Blot (WB). GTX112284
Anti-SRP1 antibody used in Western Blot (WB). GTX112284
Anti-SRP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112284

Cat No. GTX112284

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037321

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

karyopherin subunit alpha 1 , IPOA5 , NPI-1 , RCH2 , SRP1

Cellular Localization

Cytoplasm , Nucleus

Background

Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SRP1 antibody used in Western Blot (WB). GTX112284

GTX112284 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX112284 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SRP1 antibody used in Western Blot (WB). GTX112284

GTX112284 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX112284 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SRP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112284

GTX112284 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SRP1 (GTX112284) antibody at 1:200 dilution.

REFERENCE

REVIEW

SRP1 antibody Cat No. GTX112284

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Apr-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NSC-34 cells
Blocking : 3% Noraml Goat Serum, 25°C, 1Hr
Fixation : methanol
Permeabilization /Antigen retrieval : 0.1% Triton X-100/Noraml Goat Serum
Primary Antibody : 1:200, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169