GeneTex
United States (US)

SRP14 antibody [N1C3]

KO/KD
GTX101867_39596_20181123_WB_KO_watermark_19011519_214.jpg
GTX101867_WB_19011506_824.jpg

Cat No. GTX101867

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SRP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Recognition Particle 14,Alurbp,Srp14

Cellular Localization

Cytoplasm

Background

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.

Database

DATA IMAGES
GTX101867_39596_20181123_WB_KO_watermark_19011519_214.jpg

GTX101867 WB Image

KO/KD

Wild-type (WT) and SRP14 knockout (KO) 293T cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SRP14 antibody [N1C3] (GTX101867) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101867_WB_19011506_824.jpg

GTX101867 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
15% SDS PAGE
GTX101867 diluted at 1:1500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169