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SSA1 antibody [C1C3]

Comparison Orthogonal Overexpression
Anti-SSA1 antibody [C1C3] used in Western Blot (WB). GTX113554
Anti-SSA1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113554
Anti-SSA1 antibody [C1C3] used in Western Blot (WB). GTX113554
Anti-SSA1 antibody [C1C3] used in Immunoprecipitation (IP). GTX113554
Anti-SSA1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113554

Cat. No. GTX113554

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all SSA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

TRIM21-transfected 293T

Predict Reactivity

Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SSA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038037

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 21 , RNF81 , RO52 , Ro/SSA , SSA , SSA1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SSA1 antibody [C1C3] used in Western Blot (WB). GTX113554

GTX113554 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SSA1 antibody [C1C3] (GTX113554) diluted at 1:2000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SSA1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113554

GTX113554 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using SSA1(GTX113554) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SSA1 antibody [C1C3] used in Western Blot (WB). GTX113554

GTX113554 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SSA1 antibody [C1C3] (GTX113554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SSA1 antibody [C1C3] used in Immunoprecipitation (IP). GTX113554

GTX113554 IP Image

Immunoprecipitation of SSA1 protein from 293T whole cell extracts using 5 μg of SSA1 antibody [C1C3] (GTX113554).
Western blot analysis was performed using SSA1 antibody [C1C3] (GTX113554).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SSA1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113554

GTX113554 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SSA1(GTX113554) antibody at 1:200 dilution.

REFERENCE

Application Reference

Tsai YS et al. Cancer Sci 2019; 110 (4) : 1208-1219 Prothymosin-α enhances phosphatase and tensin homolog expression and binds with tripartite motif-containing protein 21 to regulate Kelch-like ECH-associated protein 1/nuclear factor erythroid 2-related factor 2 signaling in human bladder cancer.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169