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SSB antibody [1E11]

Overexpression
Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558
Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558
Anti-SSB antibody [1E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83558
Anti-SSB antibody [1E11] used in Immunoprecipitation (IP). GTX83558
Anti-SSB antibody [1E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83558
Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558
Anti-SSB antibody [1E11] used in Flow cytometry (FACS). GTX83558
Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558

Cat. No. GTX83558

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E11

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all SSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:100
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small RNA binding exonuclease protection factor La , LARP3 , La , La/SSB

Cellular Localization

Nucleus

Background

Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.

Database

Research Area

DATA IMAGES

Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558

GTX83558 WB Image

WB analysis of various samples using GTX83558 SSB antibody [1E11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558

GTX83558 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83558 SSB antibody [1E11].
Loading : 10 ug per lane
Dilution : 1:1000

Anti-SSB antibody [1E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83558

GTX83558 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SSB plasmid using GTX83558 SSB antibody [1E11].

Anti-SSB antibody [1E11] used in Immunoprecipitation (IP). GTX83558

GTX83558 IP Image

IP analysis of DDDDK tagged SSB overexpressed HEK293T lysate using GTX83558 SSB antibody [1E11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-SSB antibody [1E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83558

GTX83558 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX83558 SSB antibody [1E11].

Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558

GTX83558 WB Image

WB analysis of various cell lines using GTX83558 SSB antibody [1E11].
Loading : 35 ug per lane

Anti-SSB antibody [1E11] used in Flow cytometry (FACS). GTX83558

GTX83558 FACS Image

FACS analysis of Jurkat cells using GTX83558 SSB antibody [1E11].
Red : Primary antibody
Blue : Negative control antibody

Anti-SSB antibody [1E11] used in Western Blot (WB). GTX83558

GTX83558 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SSB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83558 SSB antibody [1E11].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519