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SSB antibody [9D6]

Overexpression
Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557
Anti-SSB antibody [9D6] used in Flow cytometry (FACS). GTX83557
Anti-SSB antibody [9D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83557
Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557
Anti-SSB antibody [9D6] used in Western Blot (WB). GTX83557
Anti-SSB antibody [9D6] used in Western Blot (WB). GTX83557
Anti-SSB antibody [9D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83557
Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557
Anti-SSB antibody [9D6] used in Immunoprecipitation (IP). GTX83557
Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557
Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

Cat. No. GTX83557

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9D6

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all SSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-2000
ICC/IF 1:50-1:100
IHC-P 1:150
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small RNA binding exonuclease protection factor La , LARP3 , La , La/SSB

Cellular Localization

Nucleus

Background

Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.

Database

Research Area

DATA IMAGES

Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

GTX83557 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83557 SSB antibody [9D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SSB antibody [9D6] used in Flow cytometry (FACS). GTX83557

GTX83557 FACS Image

FACS analysis of Jurkat cells using GTX83557 SSB antibody [9D6].
Red : Primary antibody
Blue : Negative control antibody

Anti-SSB antibody [9D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83557

GTX83557 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SSB plasmid using GTX83557 SSB antibody [9D6].

Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

GTX83557 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83557 SSB antibody [9D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SSB antibody [9D6] used in Western Blot (WB). GTX83557

GTX83557 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SSB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83557 SSB antibody [9D6].
Loading : 5 ug per lane

Anti-SSB antibody [9D6] used in Western Blot (WB). GTX83557

GTX83557 WB Image

WB analysis of various cell lines using GTX83557 SSB antibody [9D6].
Loading : 35 ug per lane

Anti-SSB antibody [9D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83557

GTX83557 ICC/IF Image

ICC/IF analysis of A549 cells using GTX83557 SSB antibody [9D6].

Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

GTX83557 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83557 SSB antibody [9D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SSB antibody [9D6] used in Immunoprecipitation (IP). GTX83557

GTX83557 IP Image

IP analysis of DDDDK tagged SSB overexpressed HEK293T lysate using GTX83557 SSB antibody [9D6].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

GTX83557 IHC-P Image

IHC-P analysis of human lung tissue using GTX83557 SSB antibody [9D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SSB antibody [9D6] used in IHC (Paraffin sections) (IHC-P). GTX83557

GTX83557 IHC-P Image

IHC-P analysis of human breast tissue using GTX83557 SSB antibody [9D6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519