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SSB antibody

Anti-SSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32896
Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX32896
Anti-SSB antibody used in Western Blot (WB). GTX32896
Anti-SSB antibody used in Immunoprecipitation (IP). GTX32896
Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX32896

Cat. No. GTX32896

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399)
See all SSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small RNA binding exonuclease protection factor La , LARP3 , La , La/SSB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-SSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32896

GTX32896 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32896 SSB antibody.
Blue : DAPI
Dilution : 1:100

Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX32896

GTX32896 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32896 SSB antibody.
Dilution : 1:100

Anti-SSB antibody used in Western Blot (WB). GTX32896

GTX32896 WB Image

WB analysis of various sample lysates using GTX32896 SSB antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SSB antibody used in Immunoprecipitation (IP). GTX32896

GTX32896 IP Image

IP analysis of Jurkat cell lysate using GTX32896 SSB antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX32896

GTX32896 IHC-P Image

IHC-P analysis of human prostate cancer tissue using GTX32896 SSB antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399