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SSEA-5 antibody [8e11]

Anti-SSEA-5 antibody [8e11] used in Flow cytometry (FACS). GTX70019
Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

Cat No. GTX70019

Host

Mouse

Clonality

Monoclonal

Clone Name

8e11

Isotype

IgG

Application

ICC/IF, FACS, IHC

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:50-1:1000
FACS 1:50
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

SSEA-5

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Undifferentiated human embryonic stem cells

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Stage-Specific Embryonic Antigen-5, SSEA-5

Cellular Localization

Cell membrane

Background

Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures.

DATA IMAGES

Anti-SSEA-5 antibody [8e11] used in Flow cytometry (FACS). GTX70019

GTX70019 FACS Image

Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.

Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

GTX70019 ICC/IF Image

Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.

Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

GTX70019 ICC/IF Image

Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution).
(A) Brightfield image
(B) Dapi
(C) anti-SSEA-5 Ab
(D) Merged image of anti-SSEA-5 Ab and DAPI

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169