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SSEA-5 antibody [8e11]

Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
Anti-SSEA-5 antibody [8e11] used in Flow cytometry (FACS). GTX70019
Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

Cat. No. GTX70019

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8e11

Isotype

 IgG

Application

 ICC/IF, FACS, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SSEA-5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:50-1:1000
FACS 1:50
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Undifferentiated human embryonic stem cells

Purification

Protein G affinity purified

Conjugation

Unconjugated

RRID

AB_11165050

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Stage-Specific Embryonic Antigen-5 , SSEA-5

Cellular Localization

Cell membrane

Background

Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures.

Research Area

DATA IMAGES

Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

GTX70019 ICC/IF Image

Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution).
(A) Brightfield image
(B) Dapi
(C) anti-SSEA-5 Ab
(D) Merged image of anti-SSEA-5 Ab and DAPI

Anti-SSEA-5 antibody [8e11] used in Flow cytometry (FACS). GTX70019

GTX70019 FACS Image

Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.

Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019

GTX70019 ICC/IF Image

Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.

REFERENCE

Application Reference

Su YH et al. Oncotarget 2018; 9 (29) : 20490-20507 A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids.
Cassidy L et al. J Biomark 2013; 2013 : 960862 Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells.
Application : ICC/IF
Reactivity : Human

Clone Reference

Tang C et al. Nat Biotechnol 2011; 29 (9):829-34 An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells.
Application : IHC
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations