APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
PC-3 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SSH3BP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885306
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
abl interactor 1 , ABI-1 , ABLBP4 , E3B1 , NAP1BP , SSH3BP , SSH3BP1
Cellular Localization
Cytoplasm , Nucleus , Cell projection , lamellipodium , filopodium , growth cone , Cell junction , synapse , synaptosome , Cytoplasm , cytoskeleton
Background
The protein encoded by this gene has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX111478 WB Image
Sample (30 ug of whole cell lysate) A: PC-3 10% SDS PAGE GTX111478 diluted at 1:1000
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GTX111478 WB Image
SSH3BP1 antibody detects ABI1 protein by Western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg BCL-1 whole cell lysate/extract | C. 30 μg Raw264.7 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract 10 % SDS-PAGE SSH3BP1 antibody (GTX111478) dilution: 1:1000
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REFERENCE
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REVIEW
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