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SSR3 antibody

Anti-SSR3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103
Anti-SSR3 antibody used in Western Blot (WB). GTX12103

Cat No. GTX12103

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

21.08 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

2.5% BSA, 0.45% NaCl, 0.1% Na₂HPO₄, 0.025% Thimerosal, 0.025% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3(8-23aa KQQSEEDLLLQDFSRN), identical to the related rat and mouse sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Endoplasmic reticulum membrane

Background

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR is comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. The first two are glycosylated subunits and the latter two are non-glycosylated subunits. This gene encodes the gamma subunit, which is predicted to span the membrane four times. [provided by RefSeq, Aug 2010]

Database

DATA IMAGES

Anti-SSR3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12103

GTX12103 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX12103 SSR3 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of human prostatic cancer tissue using GTX12103 SSR3 antibody.

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX12103 SSR3 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX12103 SSR3 antibody.

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX12103 SSR3 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX12103 SSR3 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-SSR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX12103

GTX12103 IHC-P Image

IHC-P analysis of rat brain tissue using GTX12103 SSR3 antibody.

Anti-SSR3 antibody used in Western Blot (WB). GTX12103

GTX12103 WB Image

WB analysis of various samples using GTX12103 SSR3 antibody.
Lane 1 : rat liver tissue lysate at 50ug
Lane 2 : rat spleen tissue lysate at 50ug
Lane 3 : A431 whole cell lysate at 40ug
Lane 4 : HeLa whole cell lysate at 40ug
Lane 5 : U87 whole cell lysate at 40ug
Lane 6 : SMMC whole cell lysate at 40ug
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 359