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SSU72 antibody [N1C3]

Anti-SSU72 antibody [N1C3] used in Western Blot (WB). GTX116436
Anti-SSU72 antibody [N1C3] used in Western Blot (WB). GTX116436
Anti-SSU72 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116436

Cat. No. GTX116436

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SSU72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse placenta

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SSU72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163796

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SSU72 homolog, RNA polymerase II CTD phosphatase , HSPC182 , PNAS-120

Cellular Localization

Nucleus , Cytoplasm

Background

May be involved in the C-terminal domain of RNA polymerase II dephosphorylation, RNA processing and termination.

Database

Research Area

DATA IMAGES

Anti-SSU72 antibody [N1C3] used in Western Blot (WB). GTX116436

GTX116436 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SSU72 antibody [N1C3] (GTX116436) diluted at 1:1000.

Anti-SSU72 antibody [N1C3] used in Western Blot (WB). GTX116436

GTX116436 WB Image

SSU72 antibody detects SSU72 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SSU72 antibody (GTX116436) diluted at 1:500.

Anti-SSU72 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116436

GTX116436 IHC-P Image

SSU72 antibody [N1C3] detects SSU72 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
SSU72 antibody [N1C3] (GTX116436) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chen Y et al. Genes Dev 2014; 28 (20) : 2261-75 A gene-specific role for the Ssu72 RNAPII CTD phosphatase in HIV-1 Tat transactivation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169