SSX2 antibody [4A11]

Cat. No. GTX83554

Host	Mouse
Clonality	Monoclonal
Clone Name	4A11
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all SSX2 products

Datasheet
Document

View product citations for antibody GTX83554 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SSX2 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SSX family member 2 , CT5.2 , CT5.2A , HD21 , HOM-MEL-40 , SSX

Cellular Localization

Nucleus

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 727837, located about 45 kb downstream in the opposite orientation on chromosome X. [provided by RefSeq, Jul 2013]

Database

Gene ID: 6757 SSX2
UniProt: Q16385 SSX2

Research Area

DATA IMAGES

GTX83554 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SSX2 plasmid using GTX83554 SSX2 antibody [4A11].

GTX83554 WB Image

WB analysis of HEK293T cells transfected with SSX2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83554 SSX2 antibody [4A11].
Loading : 5 ug per lane

GTX83554 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of prostate tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of endometrium tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of colon tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83554 IHC-P Image

IHC-P analysis of ovary tissue using GTX83554 SSX2 antibody [4A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

SSX2 antibody [4A11]

Cat. No. GTX83554

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83554 ICC/IF Image

GTX83554 WB Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

GTX83554 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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