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ST3GAL5 antibody [N3C3]

Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152
Anti-ST3GAL5 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115152
Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152
Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152

Cat No. GTX115152

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji , mouse brain , U87-MG

Predict Reactivity

Rat, Sheep, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ST3GAL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621350

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ST3 beta-galactoside alpha-2,3-sialyltransferase 5 , SATI , SIAT9 , SIATGM3S , SPDRS , ST3Gal V , ST3GalV

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152

GTX115152 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115152 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ST3GAL5 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115152

GTX115152 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ST3GAL5(GTX115152) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152

GTX115152 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115152 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ST3GAL5 antibody [N3C3] used in Western Blot (WB). GTX115152

GTX115152 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ST3GAL5 antibody [N3C3] (GTX115152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169