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ST6GAL1 antibody [HL2318]

Recombinant antibody Orthogonal
Anti-ST6GAL1 antibody [HL2318] used in Western Blot (WB). GTX638481
Anti-ST6GAL1 antibody [HL2318] used in IHC (Paraffin sections) (IHC-P). GTX638481
Anti-ST6GAL1 antibody [HL2318] used in IHC (Paraffin sections) (IHC-P). GTX638481
Anti-ST6GAL1 antibody [HL2318] used in Western Blot (WB). GTX638481

Cat. No. GTX638481

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2318

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ST6GAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ST6GAL1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 , SIAT1 , ST6GalI , ST6N

Cellular Localization

Secreted

Background

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-ST6GAL1 antibody [HL2318] used in Western Blot (WB). GTX638481

GTX638481 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (GTX638481) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ST6GAL1 antibody [HL2318] used in IHC (Paraffin sections) (IHC-P). GTX638481

GTX638481 IHC-P Image

ST6GAL1 antibody [HL2318] detects secreted ST6GAL1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ST6GAL1 stained by ST6GAL1 antibody [HL2318] (GTX638481) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ST6GAL1 antibody [HL2318] used in IHC (Paraffin sections) (IHC-P). GTX638481

GTX638481 IHC-P Image

ST6GAL1 antibody [HL2318] detects ST6GAL1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ST6GAL1 stained by ST6GAL1 antibody [HL2318] (GTX638481) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ST6GAL1 antibody [HL2318] used in Western Blot (WB). GTX638481

GTX638481 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (GTX638481) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations