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ST6GAL1 antibody [N2C3]

Overexpression
Anti-ST6GAL1 antibody [N2C3] used in Western Blot (WB). GTX104018
Anti-ST6GAL1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018
Anti-ST6GAL1 antibody [N2C3] used in Western Blot (WB). GTX104018
Anti-ST6GAL1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

Cat. No. GTX104018

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ST6GAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

ST6GAL1 transfected 293T

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD75. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952026

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 , SIAT1 , ST6GalI , ST6N

Cellular Localization

Secreted

Background

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Database

Research Area

DATA IMAGES

Anti-ST6GAL1 antibody [N2C3] used in Western Blot (WB). GTX104018

GTX104018 WB Image

Overexpression

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD75 antibody [N2C3] (GTX104018) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ST6GAL1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

GTX104018 IHC-P Image

CD75 antibody [N2C3] detects CD75 protein at Golgi apparatus in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
CD75 antibody [N2C3] (GTX104018) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ST6GAL1 antibody [N2C3] used in Western Blot (WB). GTX104018

GTX104018 WB Image

Western Blot analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody.
A: Non-transfected lysate.
B: ST6GAL1 transfected lysate.
12% SDS PAGE
GTX104018 diluted at 1:500

Anti-ST6GAL1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

GTX104018 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using CD75(GTX104018) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Su YH et al. Oncotarget 2018; 9 (29) : 20490-20507 A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169