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ST8SIA4 antibody

Anti-ST8SIA4 antibody used in Western Blot (WB). GTX125904
Anti-ST8SIA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX125904

Cat. No. GTX125904

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ST8SIA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Jurkat , Raji , THP-1 , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ST8SIA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 , PST , PST1 , SIAT8D , ST8SIA-IV

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ST8SIA4 antibody used in Western Blot (WB). GTX125904

GTX125904 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: THP-1
D: NCI-H929
10% SDS PAGE
GTX125904 diluted at 1:1000

Anti-ST8SIA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX125904

GTX125904 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ST8SIA4 antibody(GTX125904) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169