GeneTex
United States (US)

STAC antibody

GTX114802_40233_IHC_18121410_754.jpg
GTX114802_WB_18121410_342.jpg
GTX114802_40233_WB_M_brain_18121410_490.jpg
GTX114802_40233_IFA_18121410_806.jpg

Cat No. GTX114802

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ32331 antibody, STAC1 antibody, STAC antibody, SH3 and cysteine-rich domain-containing protein antibody, src homology three (SH3) and cysteine rich domain antibody, SRC homology 3 and cysteine-rich domain-containing protein antibody, SH3 and cysteine rich domain antibody

Cellular Localization

Cytoplasm

Background

Probably involved in a neuron-specific signal transduction.
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Database

DATA IMAGES
GTX114802_40233_IHC_18121410_754.jpg

GTX114802 IHC-P Image

STAC antibody detects STAC protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
STAC antibody (GTX114802) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114802_WB_18121410_342.jpg

GTX114802 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX114802 diluted at 1:1000

GTX114802_40233_WB_M_brain_18121410_490.jpg

GTX114802 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114802 diluted at 1:1000

GTX114802_40233_IFA_18121410_806.jpg

GTX114802 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using STAC(GTX114802) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169