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STAM antibody [N2C2], Internal

Anti-STAM antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115060
Anti-STAM antibody [N2C2], Internal used in Western Blot (WB). GTX115060
Anti-STAM antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115060
Anti-STAM antibody [N2C2], Internal used in Western Blot (WB). GTX115060
Anti-STAM antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115060

Cat. No. GTX115060

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all STAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , NCI-H929

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172707

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducing adaptor molecule , STAM-1 , STAM1

Cellular Localization

Cytoplasm (Probable) , Early endosome membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAM antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115060

GTX115060 IHC-P Image

STAM antibody [N2C2], Internal detects STAM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
STAM stained by STAM antibody [N2C2], Internal (GTX115060) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STAM antibody [N2C2], Internal used in Western Blot (WB). GTX115060

GTX115060 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: NCI-H929
10% SDS PAGE
GTX115060 diluted at 1:1000

Anti-STAM antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115060

GTX115060 ICC/IF Image

STAM antibody [N2C2], Internal detects STAM protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAM stained by STAM antibody [N2C2], Internal (GTX115060) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-STAM antibody [N2C2], Internal used in Western Blot (WB). GTX115060

GTX115060 WB Image

Whole cell extract (30 μg) was separated by -% SDS-PAGE, and the membrane was blotted with STAM antibody [N2C2], Internal (GTX115060) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAM antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX115060

GTX115060 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using STAM(GTX115060) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chou SF et al. PLoS Pathog 2015; 11 (10) : e1005123 The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169