Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX105927 diluted at 1:3000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody (GTX105927) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded AdCA xenograft, using STAM(GTX105927) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using STAM antibody (GTX105927) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX105927 diluted at 1:2000