GeneTex
United States (US)

STAP2 antibody [C3], C-term

GTX106287_39701_IHC_19011506_813.jpg
GTX106287_WB_19011506_801.jpg
GTX106287_39701_IFA_19011506_211.jpg

Cat No. GTX106287

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HCT116
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BKS antibody, FLJ20234 antibody, STAP2 antibody, breast tumor kinase substrate antibody, signal-transducing adaptor protein-2 antibody, brk kinase substrate antibody, BRK substrate antibody, signal-transducing adaptor protein 2 antibody, signal transducing adaptor family member 2 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX106287_39701_IHC_19011506_813.jpg

GTX106287 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using STAP2(GTX106287) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106287_WB_19011506_801.jpg

GTX106287 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 10% SDS PAGE
GTX106287 diluted at 1:1000

GTX106287_39701_IFA_19011506_211.jpg

GTX106287 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using STAP2(GTX106287) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169