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STAT1 antibody

Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344
Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344
Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344
Anti-STAT1 antibody used in Western Blot (WB). GTX64344
Anti-STAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64344
Anti-STAT1 antibody used in Immunoprecipitation (IP). GTX64344

Cat. No. GTX64344

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all STAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344

GTX64344 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX64344 STAT1 antibody.
Dilution : 1:100

Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344

GTX64344 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX64344 STAT1 antibody.
Dilution : 1:100

Anti-STAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64344

GTX64344 IHC-P Image

IHC-P analysis of rat lymph node tissue using GTX64344 STAT1 antibody.
Dilution : 1:100

Anti-STAT1 antibody used in Western Blot (WB). GTX64344

GTX64344 WB Image

WB analysis of HeLa cell lysate using GTX64344 STAT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-STAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64344

GTX64344 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64344 STAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-STAT1 antibody used in Immunoprecipitation (IP). GTX64344

GTX64344 IP Image

IP analysis of HeLa cell lysate using GTX64344 STAT1 antibody.
Antibody amount : 3μg / 100μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

Wang Y et al. Aging (Albany NY) 2020; 12 (20) : 20184-20197 Interleukin-6 knockout reverses macrophage differentiation imbalance and alleviates cardiac dysfunction in aging mice.
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399