Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE (3 % BSA blocking), and the membrane was blotted with STAT1 (phospho Ser727) antibody (GTX132507) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.