APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human STAT1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF3 , STAT1 , STAT91
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/templates/default/js/lightbox/images/loading.gif "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
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GTX00959 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT1 (phospho Ser701) antibody [GT1197] (GTX00959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00959 WB Image
WB analysis of PC-12 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197].
Dilution : 1:1000
Loading : 25μg
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GTX00959 WB Image
WB analysis of NIH-3T3 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197].
Dilution : 1:1000
Loading : 25μg
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GTX00959 WB Image
WB analysis of HeLa cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197].
Dilution : 1:1000
Loading : 25μg
|
REFERENCE
There are currently no references for STAT1 (phospho Tyr701) antibody [GT1197] (GTX00959). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STAT1 (phospho Tyr701) antibody [GT1197] (GTX00959). Be the first to share your experience with this product.
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/small/GTX00959/GTX00959_4000000049_20200313_WB_treatment_hIFN-alpha_w_23053121_963.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/small/GTX00959/GTX00959_20200327_WB_2_w_23053121_771.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/small/GTX00959/GTX00959_20200327_WB_3_w_23053121_895.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/small/GTX00959/GTX00959_20200327_WB_1_w_23053121_463.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/normal/GTX00959/GTX00959_4000000049_20200313_WB_treatment_hIFN-alpha_w_23053121_963.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_2_w_23053121_771.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_3_w_23053121_895.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")
![Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959](/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_1_w_23053121_463.webp "Anti-STAT1 (phospho Tyr701) antibody [GT1197] used in Western Blot (WB). GTX00959")