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STAT1 (phospho Tyr701) antibody

Anti-STAT1 (phospho Tyr701) antibody used in Western Blot (WB). GTX50118
Anti-STAT1 (phospho Tyr701) antibody used in Western Blot (WB). GTX50118
Anti-STAT1 (phospho Tyr701) antibody used in IHC (Paraffin sections) (IHC-P). GTX50118

Cat No. GTX50118

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from human STAT1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STAT1 (phospho Tyr701) antibody used in Western Blot (WB). GTX50118

GTX50118 WB Image

WB analysis of extracts from MEF cells untreated or treated with interferon-ɤ (IFNɤ) using GTX50118 STAT1 (phospho Tyr701) antibody.

Anti-STAT1 (phospho Tyr701) antibody used in Western Blot (WB). GTX50118

GTX50118 WB Image

WB analysis of extracts from HeLa cells treated with IFNa or calf intestinal phosphatase (CIP) using GTX50118 STAT1 (phospho Tyr701) antibody.

Anti-STAT1 (phospho Tyr701) antibody used in IHC (Paraffin sections) (IHC-P). GTX50118

GTX50118 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50118 STAT1 (phospho Tyr701) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf