GeneTex
United States (US)

STAT2 antibody [C2C3], C-term

IP/MS
GTX103117_40688_IP_19011506_229.jpg
GTX103117_40688_20150821_IHC-P_2_19011506_478.jpg
GTX103117_40688_WB_19011506_751.jpg
GTX103117_40688_20150821_IHC-P_19011506_244.jpg

Cat No. GTX103117

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377386)

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 2,Imd44,Isgf-3,P113,Stat113,Stat2

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. [provided by RefSeq]

Database

DATA IMAGES
GTX103117_40688_IP_19011506_229.jpg

GTX103117 IP Image

STAT2 antibody immunoprecipitates STAT2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of STAT2 protein by 2 μg of STAT2 antibody (GTX103117) 7.5% SDS-PAGE The immunoprecipitated STAT2 protein was detected by STAT2 antibody (GTX103117) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103117_40688_20150821_IHC-P_2_19011506_478.jpg

GTX103117 IHC-P Image

STAT2 antibody [C2C3], C-term detects STAT2 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103117_40688_WB_19011506_751.jpg

GTX103117 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
5% SDS PAGE
GTX103117 diluted at 1:2000

GTX103117_40688_20150821_IHC-P_19011506_244.jpg

GTX103117 IHC-P Image

STAT2 antibody [C2C3], C-term detects STAT2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169