APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human STAT3 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752](/templates/default/js/lightbox/images/loading.gif "Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752")
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GTX82752 WB Image
WB analysis of HeLa (1),NIH3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate using GTX82752 STAT3 antibody [3B5].
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GTX82752 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX82752 STAT3 antibody [3B5].
Green : STAT3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX82752 IHC-P Image
IHC-P analysis of mammary cancer tissue (left) and lung cancer tissue (right) using GTX82752 STAT3 antibody [3B5].
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REFERENCE
There are currently no references for STAT3 antibody [3B5] (GTX82752). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STAT3 antibody [3B5] (GTX82752). Be the first to share your experience with this product.
![Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752](/upload/website/prouct_img/small/GTX82752/GTX82752_20170912_WB_w_23061322_524.webp "Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752")
![Anti-STAT3 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82752](/upload/website/prouct_img/small/GTX82752/GTX82752_20170912_ICCIF_w_23061322_468.webp "Anti-STAT3 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82752")
![Anti-STAT3 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX82752](/upload/website/prouct_img/small/GTX82752/GTX82752_20170912_IHC-P_w_23061322_909.webp "Anti-STAT3 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX82752")
![Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752](/upload/website/prouct_img/normal/GTX82752/GTX82752_20170912_WB_w_23061322_524.webp "Anti-STAT3 antibody [3B5] used in Western Blot (WB). GTX82752")
![Anti-STAT3 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82752](/upload/website/prouct_img/normal/GTX82752/GTX82752_20170912_ICCIF_w_23061322_468.webp "Anti-STAT3 antibody [3B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82752")
![Anti-STAT3 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX82752](/upload/website/prouct_img/normal/GTX82752/GTX82752_20170912_IHC-P_w_23061322_909.webp "Anti-STAT3 antibody [3B5] used in IHC (Paraffin sections) (IHC-P). GTX82752")