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STAT3 antibody [3F4]

Anti-STAT3 antibody [3F4] used in Western Blot (WB). GTX34234
Anti-STAT3 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX34234
Anti-STAT3 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX34234

Cat No. GTX34234

Host Mouse
Clonality Monoclonal
Clone Name 3F4
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Specificity/Sensitivity

STAT3 Mouse Monoclonal Antibody (3F4) detects endogenous levels of STAT3
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 3,Admio,Admio1,Aprf,Hies,Stat3

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-STAT3 antibody [3F4] used in Western Blot (WB). GTX34234

GTX34234 WB Image

Western Blot (WB) analysis of 1) Rat Heart Tissue, 2)Mouse Heart Tissue, 3) HeLa with STAT3 Mouse Monoclonal Antibody diluted at 1:2000.

Anti-STAT3 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX34234

GTX34234 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Brain Tissue using STAT3 Mouse Monoclonal Antibody diluted at 1:200.

Anti-STAT3 antibody [3F4] used in IHC (Paraffin sections) (IHC-P). GTX34234

GTX34234 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon Carcinoma using STAT3 Mouse Monoclonal Antibody diluted at 1:200.