ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX15789 STAT3 antibody [9D8]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

IHC-P analysis of biopsies of normal and cancer tissues using GTX15789 STAT3 antibody [9D8].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95ºC
Dilution : 1:1600

IP analysis of HepG2 cell lysates using GTX15789 STAT3 antibody [9D8].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:5000

ICC/IF analysis of HeLa cells using GTX15789 STAT3 antibody [9D8].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

WB analysis of STAT3 siRNA transfected or non-targeting control transfected U2OS cell lysate using GTX15789 STAT3 antibody [9D8].
Dilution : 1:1000
Loading : 25 μg