GeneTex
United States (US)

STAT3 antibody [9D8]

KO/KD Orthogonal
GTX15789_6_ChIP_19011519_710.jpg
GTX15789_1538_WB_19011519_618.jpg
GTX15789_2042_IHC-P_19011519_814.jpg
GTX15789_317_ICC-IF_19011519_824.jpg
GTX15789_1429_IP_19011519_853.jpg

Cat No. GTX15789

Host Mouse
Clonality Monoclonal
Clone Name 9D8
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000
ICC/IF 1:100
IHC-P 1:1600
IP 2 μg
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

88 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Residues 655-770 from human recombinant protein expressed in bacteria

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 3,Admio,Admio1,Aprf,Hies,Stat3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
GTX15789_6_ChIP_19011519_710.jpg

GTX15789 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX15789 STAT3 antibody [9D8]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

GTX15789_1538_WB_19011519_618.jpg

GTX15789 WB Image

KO/KD

WB analysis of STAT3 siRNA transfected or non-targeting control transfected U2OS cell lysate using GTX15789 STAT3 antibody [9D8].
Dilution : 1:1000
Loading : 25 µg

GTX15789_2042_IHC-P_19011519_814.jpg

GTX15789 IHC-P Image

IHC-P analysis of biopsies of normal and cancer tissues using GTX15789 STAT3 antibody [9D8].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C
Dilution : 1:1600

GTX15789_317_ICC-IF_19011519_824.jpg

GTX15789 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15789 STAT3 antibody [9D8].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

GTX15789_1429_IP_19011519_853.jpg

GTX15789 IP Image

IP analysis of HepG2 cell lysates using GTX15789 STAT3 antibody [9D8].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:5000

REFERENCE