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STAT3 antibody

KO/KD Comparison Orthogonal IP/MS
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Anti-STAT3 antibody used in Flow cytometry (FACS). GTX108630

Cat. No. GTX108630

Reference 4
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, IP

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all STAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377393)

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT3 antibody used in Western Blot (WB). GTX108630

GTX108630 WB Image

KO/KD

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody (GTX108630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody used in Western Blot (WB). GTX108630

GTX108630 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 antibody (GTX108630) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-STAT3 antibody used in Western Blot (WB). GTX108630

GTX108630 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody (GTX108630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-STAT3 antibody used in Western Blot (WB). GTX108630

GTX108630 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody (GTX108630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody used in Flow cytometry (FACS). GTX108630

GTX108630 FACS Image

The data was published in the journal Cancer Cell Int in 2015. PMID: 25805962

REFERENCE

Application Reference

L?pez-Mej?a JA et al. Int J Mol Sci 2022; 23 (15) c-Kit Induces Migration of Triple-Negative Breast Cancer Cells and Is a Promising Target for Tyrosine Kinase Inhibitor Treatment.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Uchino Y et al. Cancer Cell Int 2015; 15 : 30 Granulocyte colony-stimulating factor potentiates all-trans retinoic acid-induced granulocytic differentiation in acute promyelocytic leukemia cell line HT93A.
Submit a Reference

REVIEW

STAT3 antibody Cat. No. GTX108630

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Date : Anonymous submitted on 13-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC3 cells
Amount used : 30μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: OC3 cells, Lane 2: knockdown of human STAT3 in OC3 cells
Anti-STAT3 antibody used in Western Blot (WB). GTX108630
Date : Anonymous submitted on 06-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC-15 cells
Amount used : 15μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169