GeneTex
United States (US)

STAT3 antibody

GTX108630_39988_20170504_WB_Z_eye_19011507_986.jpg
GTX108630_39988_IFA_19011507_266.jpg

Cat No. GTX108630

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Rat, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

zebrafish eye

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377393)

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Japanese Medaka(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 3,Admio,Admio1,Aprf,Hies,Stat3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108630_39988_20170504_WB_Z_eye_19011507_986.jpg

GTX108630 WB Image

Zebrafish tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody (GTX108630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108630_39988_IFA_19011507_266.jpg

GTX108630 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using STAT3(GTX108630) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
REVIEW

STAT3 antibody Cat No. GTX108630

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 13-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC3 cells
Amount used : 30μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : Lane 1: OC3 cells, Lane 2: knockdown of human STAT3 in OC3 cells
Date : Anonymous submitted on 06-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC-15 cells
Amount used : 15μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf