GeneTex
United States (US)

STAT3 antibody

Anti-STAT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31870
Anti-STAT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31870
Anti-STAT3 antibody used in Western Blot (WB). GTX31870

Cat No. GTX31870

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

Human Small Intestine Tissue Lysate(GTX27923) , Human Small Intestine Tissue Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal STAT3 antibody was raised against a 19amino acid peptide near the amino terminus of human STAT3.The immunogen is located within amino acids 170 - 220 of STAT3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Transducer And Activator Of Transcription 3,Admio,Admio1,Aprf,Hies,Stat3

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-STAT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31870

GTX31870 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31870 STAT3 antibody.
Working concentration : 20 μg/ml

Anti-STAT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31870

GTX31870 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31870 STAT3 antibody.
Working concentration : 5 μg/ml

Anti-STAT3 antibody used in Western Blot (WB). GTX31870

GTX31870 WB Image

WB analysis of human small intestine tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31870 STAT3 antibody.
Working concentration : 1 μg/ml