Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Ser727) antibody [GT1206] (GTX00968) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of NIH-3T3 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00968 STAT3 (phospho Ser727) antibody [GT1206].
Dilution : 1:1000
Loading : 25μg

IHC-P analysis of human lung cancer tissue section using GTX00968 STAT3 (phospho Ser727) antibody [GT1206].
Dilution : 1:100

WB analysis of PC-12 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30 min) treatment using GTX00968 STAT3 (phospho Ser727) antibody [GT1206].
Dilution : 1:1000
Loading : 25μg

IHC-P analysis of rat spleen tissue section using GTX00968 STAT3 (phospho Ser727) antibody [GT1206].
Dilution : 1:100

IHC-P analysis of mouse kidney tissue section using GTX00968 STAT3 (phospho Ser727) antibody [GT1206].
Dilution : 1:100