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STAT3 (phospho Ser727) antibody

Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in IHC (Paraffin sections) (IHC-P). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50120

Cat No. GTX50120

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from human STAT3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120

GTX50120 WB Image

WB analysis of extracts from HeLa and HepG2 cells untreated or treated with PMA using GTX50120 STAT3 (phospho Ser727) antibody.

Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120

GTX50120 WB Image

WB analysis of extracts from HeLa cells treated with IFNa or calf intestinal phosphatase (CIP) using GTX50120 STAT3 (phospho Ser727) antibody.

Anti-STAT3 (phospho Ser727) antibody used in IHC (Paraffin sections) (IHC-P). GTX50120

GTX50120 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50120 STAT3 (phospho Ser727) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-STAT3 (phospho Ser727) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50120

GTX50120 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50120 STAT3 (phospho Ser727) antibody.

REFERENCE

There are currently no references for STAT3 (phospho Ser727) antibody (GTX50120). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for STAT3 (phospho Ser727) antibody (GTX50120). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf