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STAT3 (phospho Tyr705) antibody [HL1464]

Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938
Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938
Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938
Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938

Cat. No. GTX636938

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1464

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Mouse, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr705 of human STAT3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938

GTX636938 WB Image

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody [HL1464] (GTX636938) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938

GTX636938 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 (phospho Tyr705) antibody [HL1464] (GTX636938) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938

GTX636938 WB Image

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody [HL1464] (GTX636938) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-STAT3 (phospho Tyr705) antibody [HL1464] used in Western Blot (WB). GTX636938

GTX636938 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody [HL1464] (GTX636938) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for STAT3 (phospho Tyr705) antibody [HL1464] (GTX636938). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations